通过同源重组,一个380bp包含起始翻译位点的片段被替换了 neo cassette。 Western blot结果显示,纯合小鼠大脑匀浆中未检测到完整和剪切片段的蛋白质(来源:J:113647)
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A 380 bp fragment including the translation start site was replaced with a neo cassette via homologous recombination. Western blot confirmed absence of full length and truncated protein in whole brain homogenates from homozygous mice. (J:113647)