前三个外显子被替换成BFP基因和一个敲除neo cassette,通过脑组织提取物的 Western blot 实验确认了蛋白表达的缺失。(来源:J:111540)
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The first three exons were replaced with a BFP gene and a floxed neo cassette. The absence of protein expression was confirmed by western blot analysis on brain extracts. (J:111540)