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An IRES element, eYFP and a bovine polyA sequence followed by a floxed neomycin resistance cassette were inserted into the end of the coding sequence. Targeting took place in ES cells homozygous for Tg(Prm-cre)70Og, which express Cre recombinase in male germ cells. Cre-mediated excision subsequently removed the neomycin cassette upon transmission from male chimeric mice. The protamine-cre transgene was separated from the knock-in allele by crossing germline male mutant mice with C57BL/6 or BALB/c mice. (J:113265)