将靶向载体插入PC3 ES细胞后,在内含子2位置插入了loxP位点,并在内含子5插入了含floxed neo抗性元件的 cassette。通过Cre介导的剪切,在雄性杂合体传代时,去除了exons 3-5。免疫印迹证实了突变小鼠中蛋白的缺失。(来源:J:112395)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S4/SvJae-Tg(Prm-cre)70Og
Targeted
插入,基因内删除
--
1
--
3

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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