将靶向载体插入PC3 ES细胞后,在内含子2位置插入了loxP位点,并在内含子5插入了含floxed neo抗性元件的 cassette。通过Cre介导的剪切,在雄性杂合体传代时,去除了exons 3-5。免疫印迹证实了突变小鼠中蛋白的缺失。(来源:J:112395)
Basic Information
129S4/SvJae-Tg(Prm-cre)70Og
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count