A Beta-geo gene trap vector, pGT1Lxf, was inserted into intron 13 of the locus. The first 285 amino acids were fused to the Beta-galactosidase-neomycin protein. This allele retains most of the proline-rich stretch as it loses only the C-terminal 103 residues. Western blots demonstrate expression of chimeric protein composed of the endogenous N-terminal polypeptide fused to Beta-geo. (J:111801)