设计了一个靶向载体,用于在内源基因(exon 8的终止密码子下游)插入增强型绿色荧光蛋白(EGFP,由Clontech提供,带有牛生长激素poly(A)信号和loxP介导的Neo抗性基因座)。构建物通过电穿孔法导入了含Tg(Prm-cre)70Og转基因的ES细胞,该转基因在雄性生殖细胞中通过Cre介导的重组能消除Neo cassette。来源:(J:112745)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S4/SvJae-Tg(Prm-cre)70Og
Targeted
插入
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1
2
3

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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