设计了一种向量,用于替换包含ATG的片段,替换为诺卡因抗性基因座。 Western blot在突变的MEFs中未能检测到蛋白质。(来源:J:111404)
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A vector was designed to replace a fragment encompassing the ATG with a neomycin resistance cassette. Western blot failed to detect protein in mutant MEFs. (J:111404)