设计了一个靶向载体,用于替换1和2号外显子,替换为诺卡因抗性基因座。 Western blot结果显示突变体中没有蛋白表达(来源:J:111395)
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A targeting vector was designed to replace exons 1 and 2 with a neomycin resistance cassette. Western blot analysis demonstrated absence of protein in mutants. (J:111395)