Gabra1^tm3Geh

The 3' end was modified to flank exons 9 and 10 wit loxP sites, insert a mutated duplication of exons 9 and 10 downstream of all alpha1 coding and regulatory sequences and insert a neo cassette flanked by frt sites. Exons 9 and 10 were fused together and intron 9 deleted in the vector. The knockin mutations for exon 9 were to change serine 270 to histidine and leucine 277 to alanine. The neo was subsequently removed via crossing with FLPe general deleter mice. (J:110982)