Exon 2周围有loxP位点,连接了一个人类EFNB1的cDNA,带有polyA信号。 Western blot检测到了突变体中的蛋白质。通过flipase表达,去除了耐药性基因Neo。(来源:J:110732)
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Exon 2 was flanked by loxP sites and fused to a human EFNB1 cDNA with a polyA signal. Western blot detected protein from mutants. A neomycin resistance gene was removed via flipase expression. (J:110732)