一种新霉素抗性基因替换了一个exon 2,导致产生了框移突变,这种突变会破坏蛋白质的除了前10个氨基酸以外的所有部分。 Western blot结果显示突变体脾脏中没有检测到蛋白质(来源:J:33052)
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A neomycin resisitance gene replaced exon 2 such that the resulting frame shift mutation would disrupt all but the first 10 amino acids of the protein. Western blot confirmed absence of protein in mutant spleens. (J:33052)