Tg(CAG-cat,-EGFP)39Miya

This Cre recombinase reporter transgene employs the "CAG promoter," which consists of the cytomegalovirus immediate early (CMV-EI) enhancer followed by a 1.3-kb DNA segment including the promoter, first exon and first intron of the chicken beta-actin gene, with the 3' splice junction sequence replaced by that of the rabbit hemoglobin beta gene. The promoter is separated from the EGFP coding sequence, with a rabbit globin polyadenylation signal, by a loxP-flanked chloramphenicol acetyltransferase gene (cat) with the SV40 small t intron and polyadenylation signal. The cat gene is efficiently excised by Cre recombinase, permitting EGFP expression under control of the beta-actin promoter. (J:83047)