设计了一个靶向载体,用于替换含有第二外显子部分的0.2kb基因组片段,替换为neo cassette。 Western blot结果显示突变的MEFs中没有蛋白表达。(来源:J:110590)
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A targeting vector was designed to replace the 0.2 kb genomic fragment containing part of the second exon with a neo cassette. Western blot confirmed absence of protein in mutant MEFs. (J:110590)