随机插入的反向转录病毒基因捕获载体ROSA,带有splice接受器,包括β-半乳糖苷酶基因及其配套的splice接受器和neo序列。构建体在pGen-反向插入得到了确认。RT-PCR和 Western blot实验表明,该载体在纯合的肺和肾脏中未检测到mRNA和蛋白表达。(来源:J:110222)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count