Tg(EEF1A1-MCL1)12Jko

The transgene contains the cDNA encoding human myeloid cell leukemia sequence 1 (BCL2-related) joined to the promoter and 5' regulatory sequences of the human eukaryotic translation elongation factor 1 gene, including 1.5 kb of 5' flanking DNA, the first (noncoding) exon and the first intron. RT-PCR analysis detected expression in all organs/tissues examined; levels of expression differed among organs, being greatest in the islets of Langerhans, but was much lower everywhere than that of the endogenous gene. Levels in all tissues were too low for detection by northern blot analysis. (J:83768)