将抗氨苄青霉素抗性基因插入到了野生型小鼠的2号和3号外显子中。对突变体的 Western blot 分析未能检测到野生型小鼠中存在的多种不同剪接产物。(来源:J:77909)
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A neomycin resistance gene was inserted into exons 2 and 3. Western blot analysis of mutants failed to detect the multiple alternative splice products found in wild-type mice. (J:77909)