通过同源重组,将第一个外显子和启动子区域替换为诺卡因抗性基因。 Western blot和酶活性测定在纯合肝提取物中未检测到任何蛋白质或活性。(来源:J:110257)
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The first exon and promoter region were replaced with a neomycin resistance gene via homologous recombination. Western blot and enzyme activity assays did not detect any protein or activity in homozygous liver extracts. (J:110257)