Igh^{tm9(VHB1-8)Cgn}

Through homologous recombination a modified version of the vector used to create Ightm2Cgn replaced the endogenous sequence. This vector replaces a 3' Igh-D element (DQ52) and Igh-J elements with a recombined antibody variable region derived from a 4-hydroxy-3-nitrophenyl acetyl binding antibody (B1-8), includes a termination codon in the leader exon of B1-8 and replaces the endogenous promoter with a bipartite 2.2 kb pol I promoter fragment. (J:48835)