通过同源重组,一种经过修改的Ightm2Cgn构建载体替换了内源序列。这种载体替换了3'的Igh-D元件(DQ52)和Igh-J元件,使用了由4-羟基-3-硝基苯甲酰基结合抗体(B1-8)重组的抗体可变区,包含B1-8 leader exon的终止密码子,并去除了内源性启动子。(来源:J:48835)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
129P2/OlaHsd
Targeted
插入
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3

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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