The enhanced green fluorescent protein coding sequence was cloned into a pCAGGS expression vector downstream of the cytomegalovirus immediate early enhancer (CMV-EI) followed by the promoter, first exon and first intron of the chicken beta-actin gene; the EGFP sequence is followed by the bovine globin polyadenylation signal. Transgenic founders and their progeny were identified by whole-body fluorescence of neonates. (J:110379)