Del(16Es2el-Sept5)3Bld

A proximal loxP site was targeted into the Es2el gene. The targeting vector replaced a 200-bp fragment containing exon 4. A second loxP site was introduced into the Sept5 gene, replacing exons 2-4. Embryonic stem cells that were double-targeted in trans were subjected to transient Cre expression and underwent recombination to produce a reciprocal deletion of the Es2el-Sept5 interval. (J:67409)