在包含第一个甲硫氨酸的ATG起始密码子的exon 2中插入了抗氨苄青霉素抗性基因 cassette,导致了基因功能受损。E12.5期胚胎的提取物经 Western blot 检测并未发现蛋白质。(来源:J:108699)
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Insertion of a neomycin resistance cassette into exon 2, which contains the first methionine ATG, disrupted the gene. Western blot analysis of E12.5 embryo lysates did not detect protein. (J:108699)