设计了一个靶向载体,用于替换前三个外显子和部分5' UTR,替换为诺卡因抗性基因。对突变睾丸提取物的 Western blot 分析并未检测到蛋白质的存在。(来源:J:109004)
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A targeting vector was designed to replace the first three exons and part of the 5' UTR with a neomycin resistance gene. Western blot analysis of mutant testes extracts did not indicate presence of protein. (J:109004)