Zfhx2/Zfhx2os^tm2Ymri

A targeting vector was designed to replace exons 2-6 of Zfhx2 with that of EGFP-pA-neo, such that no functional Zfhx2 mRNA would be produced, but EGFP would be directed by the Zfhx2 promoter. Neither sense (Zfhx2) nor antisense (Zfhx2os) transcripts were expressed except for antisense exons 7-10. In situ hybridization detected ectopic expression of EGFP in the cerebral cortex and pontine nuclei, unlike wild-type expression of Zfhx2. Due to an incomplete termination at the polyA site of the neo gene, EGFP antisense transcripts were also detectable, however, at a much lower level than the wild-type antisense RNA. (J:106421)