在通过CAG-TBD-CRE-TBD转基因构建电转导入Runx1tm1Medv胚胎干细胞后,表达出Tamoxifen依赖的Cre重组酶,导致之前插入的Engrailed2-SA-lacZ序列被剔除,剩下位于基因5'UTR内的单个LoxP位点。肺和胸腺的Northern blot结果显示主要剪接异构体的正常表达。(来源:J:106769)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count