在5'UTR的下游,通过loxP引导的停泊盒插入了一个从小鼠Engrailed-2基因来的1.7kb剪接接受体(SA)序列,以阻止远端启动子产生的转录。NLS被放置在上游远端启动子外显子和Betagal基因之间,并在lacZ下游包含了转录终止元件,以防止盒内序列的跳跃转录。通过瞬时表达Cre重组酶来去除neo序列。(Engrailed2-SA-lacZ插入序列由loxP座点 flank。)(J:106769)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129P2/OlaHsd
Targeted
插入
--
1
8
8

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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