将交叉嵌合的转基因小鼠与在生殖细胞中表达Cre重组酶的鼠杂交,导致1-4号外显子被剔除。 Western blot在突变体中未能检测到蛋白质(来源:J:108574)
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Crossing floxed mice with mice expressing cre recombinase in the germline resulted in the excision of exons 1-4. Western blot failed to detect protein in mutants. (J:108574)