设计了一个靶向构建,用于替换从5'上游启动子区域到内含子3的序列,替换成诺卡因抗性基因。 Western blot在突变大脑提取物中未能检测到蛋白质。(来源:J:108765)
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A targeting construct was designed to replace sequence spanning from the 5' upstream promoter region to intron 3 with a neomycin resistance gene. Western blot failed to detect protein in mutant brain extract. (J:108765)