设计了一个靶向元件,用于替换17、18和19部分序列,这部分编码三个Ig样域,替换为PGK-neo cassette。 Western blot未能检测到主要的isoform。(来源:J:100650)
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A targeting construct was designed to replace exons 17 and 18 and part of 19, sequence encoding the three Ig-like domains, with a PGK-neo cassette. Western blot failed to detect either the main isoform. (J:100650)