设计了一个靶向元件,将7个C端的赖氨酸替换为精氨酸(p.K367R, p.K369R, p.K370R, p.K378R, p.K379R, p.K383R, p.K384R)。载体中包含了一个由loxP序列引导的诺卡因抗性基因座。这个载体通过电穿孔技术导入了携带有自我剪接Cre重组酶基因的ES细胞。(来源:J:100189)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S4/SvJae
Targeted
Insertion, Nucleotide substitutions
--
1
65
3

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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