设计了一种靶向载体,用于替换exon 1、exon 2和exon 3的编码区域,用新霉素抗性基因替换。 Western blot在突变体中未能检测到蛋白质。(来源:J:107486)
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A targeting vector was designed to replace exon 1, exon 2 and the coding part of exon 3 with a neomycin resistance gene. Western blot failed to detect protein in mutants. (J:107486)