设计了一个靶向载体,用于替换exon 4和大部分5,用PGK-neo cassette替代。删除序列编码了DNA结合区域。 Western blot结果显示突变核提取物中无蛋白表达。(来源:J:107381)
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A targeting vector was designed to replace exon 4 and most of 5 with a PGK-neo cassette. The deleted sequence encodes the DNA-binding domain. Western blot confirmed absence of protein in mutant nuclear extracts. (J:107381)