Psme4^tm1Slek

A floxed neo was inserted immediately upstream of exon 2 using one targeting vector. The ES cells were targeted again to insert a floxed neo immediately downstream of exon 48 of exon 2 using one targeting vector. Cre-recombinase interchromosomal recombination resulted in the loss of most of the locus, leaving only the first coding exon. At most, the mutant locus would encode the N-terminal 80 amino acids of the protein. Northern blot did not detect mRNA from mutants. Western blot confirmed absence of protein. (J:107376)