Exon 3被替换成一个PGK-抗氨苄青霉素抗性 cassette。在3个阅读框的外显子3剪接接受位点后引入了终止密码子。 Western blot未能在突变体中检测到蛋白质。(来源:J:106976)
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Exon 3 was replaced with a PGK-neomycin resistance cassette. Stop codons in all 3 reading frames were introduced after the exon 3 splice acceptor site. Western blot failed to detect protein in mutants. (J:106976)