Adam19^tm1Asf

A PGK-neo replaced exons 10-12. Exon 11 contains the active site of the metalloprotease domain. The construct generated a frame shift, thereby allowing mutants to express only an N-terminal truncated protein. RT-PCR failed to detect wild-type transcript in mutants. An alternatively spliced isoform lacking the metalloprotease and disintegrin domains also failed to be detected. (J:106261)