一种新霉素抗性基因替换了所有剪接变体编码主要蛋白质的第二外显子。 Western blot结果显示,包括卵巢在内的所有突变组织中,这些变体均不存在(来源:J:106320)
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A neomycin resistance gene replaced exon 2, which encodes the majority of the protein in all splice variants. Western blot confirmed that all variants were absent in mutant tissues including ovary. (J:106320)