Tg(Pbsn-AKT1)9Wrs

The transgene comprises nucleotides -421 through +28 of the rat probasin gene, containing the promoter, upstream of a cDNA encoding hemagglutinin (HA) epitope tagged, myristoylated human AKT1, the product of the human ortholog of mouse thymoma viral proto-oncogene 1. RT-PCR and immunoblot analysis with antibody to the HA tag revealed prostate-specific expression of both mRNA and protein; immunoblot analysis of protein extracts of individual prostatic lobes and other tissues from the urogenital tract localized expression to the ventral prostate and, at low levels, to the lateral prostate. (J:84509)