设计了一种靶向载体,旨在替换大部分编码区域,包括1-5号外显子,用新霉素抗性基因座替代。 Western blot在突变粒细胞中未能检测到蛋白(来源:J:106079)
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A targeting vector was designed to replace most of the coding region, including exons 1-5, with a neomycin resistance cassette. Western blot failed to detect protein in mutant neutrophils. (J:106079)