Southern blot分析和相位荧光原位杂交(FISH)都显示,来自CITB小鼠库的194kb BAC克隆551D6在129S1/Sv衍生的CJ7胚胎干细胞基础上,大约整合了40个拷贝。这个克隆包含一个187.2kb的插入,覆盖了Nr2e1基因的全长。在C57BL/6J小鼠中,与转基因等位基因纯合的脑组织mRNA,经特异性RT-PCR检测,显示在回交N10代有129S1/Sv来源的转基因等位基因的表达。(来源:J:105597)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
(C57BL/6J x 129S1/SvImJ)F1
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插入
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1
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1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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