Tg(CD2-Tgfbr2)1Grs

The transgene comprises a 0.7-kb DNA fragment encoding a truncated transforming growth factor beta type II receptor lacking the cytoplasmic tail, with a 5' influenza hemaglutinin (HA) tag, between a 5 kb human genomic DNA fragment containing the promoter through the first intron of the CD2 gene and a 5.5 kb fragment from the 3' untranslated region of human CD2 containing two polyadenylation signals and the locus control region (LCR). T-cell specific transgene mRNA expression in splenocytes of transgenic mice was demonstrated by semi-quantitative RT-PCR using primers for the HA tag sequence. Expression levels are similar in CD4+ and in CD8+ T cells. Transgene-derived, TGF beta 1-binding receptors were identified on T cells cultured from transgenic mice by incubating the cells with 125I-labeled ligand, then cross-linking the receptor-ligand complexes and precipitating them from cell lysates with HA tag-specific monoclonal antibodies. (J:97523)