将促域替换为 Xenopus Bmp2 的,带有 N-端的FLAG 或 V5 标签的成熟配体,然后合成了融合蛋白。通过 Western blot 检测到了多种蛋白质。(来源:J:104371)
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The prodomain was replaced with that of Xenopus Bmp2 with a FLAG or V5 epitope tag at the N-terminus of the mature ligand. The resulting fusion protein was expressed. Several proteins were detected by Western blot. (J:104371)