通过Cre介导的重组,去除了新霉素抗性基因 cassette,保留了exon 2的敲除。对突变肝脏的 Western blot 检测显示,蛋白的表达与野生型动物相同。(来源:J:104388)
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A neomycin resistance cassette was removed via cre-mediated recombination, leaving exon 2 floxed. Western blot of mutant livers confirmed protein was expressed as in wild-type animals. (J:104388)