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A promoterless IRES-lacZ-neo-loxP cassette replaced the translation start site and all of the first exon and about 300 bp of the first intron. Western blot analysis failed to detect protein in newborn mutants. Whole-embryo staining showed widespread expression, particularly within the peripheral nervous system, craniofacial region, dermis of the skin, and body cavities. (J:103753)