一个反向排列的诺他霉素抗性基因替换了 intron 1 的部分、exon 2,以及 intron 2 的一部分。通过 Northern blot 验证了肝脏中 mRNA 的缺失。 Western blot 在突变体中未能检测到蛋白质。(来源:J:102729)
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A neomycin resistance gene in opposite orietation replaced part of intron 1, exon 2 and part of intron 2. The absence of mRNA in livers was confirmed by Northern blot. Western blot failed to detect protein in mutants. (J:102729)