通过基因靶向策略,将HSV-tk/beta-actin-neo cassette插入了内皮2号染色体。表达这两种标记物的ES细胞被筛选出来。在对400rad照射后的正确靶向ES细胞系进行X射线处理后,通过FIAV检测tk表达的丢失。不再表达tk的细胞随后检查了neomycin表达的缺失。SSLP类型多态性微卫星标记的遗传分型显示,这一等位基因包含一个大于148kb的缺失,可能是跳跃性缺失。FISH确认了基因组序列的缺失(来源:J:102039)。

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(C57BL/6J x 129X1/SvJ)F1
Radiation induced
Deletion, Insertion
--
1
--
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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