将细菌的lacZ基因插入,使其由内源性基因驱动β-半乳糖苷酶的表达。RT-PCR检测结果显示,该基因转录在大脑、脊髓、眼、皮肤、舌和睾丸中有表达;在大脑和眼中有β-半乳糖苷酶的表达,但心脏、皮肤、舌和睾丸中未检测到。 (LacZ基因: β-半乳糖苷酶基因; RT-PCR: Real-Time Polymerase Chain Reaction; endogenous gene promoter: 内源性基因驱动; brain spinal cord, eye, heart, skin, tongue, testis: 大脑、脊髓、眼、心脏、皮肤、舌和睾丸; beta-galactosidase expression: β-半乳糖苷酶表达)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129P2/OlaHsd
Targeted
插入,基因内删除
--
1
4
6

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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