将细菌的lacZ基因插入,使其由内源性基因启动子驱动β-半乳糖苷酶的表达。RT-PCR分析显示所有测试组织中都存在基因转录,但这个等位基因在大脑、脊髓、眼睛、硬腭腺、胸腺、脾、淋巴结、主动脉、心脏、肺、肝、胆囊、胰腺、肾、膀胱、气管、喉、食管、甲状腺、垂体、肾上腺、唾液腺、舌、骨骼肌、皮肤以及男性和女性生殖系统中,都能检测到强的β-半乳糖苷酶表达。在许多组织中,lacZ在平滑肌细胞中表达。(来源:原文)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129P2/OlaHsd
Targeted
插入
--
1
2
7

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

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PMID
Journal
Year
IF
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