Chrna2^tm1Dgen

A bacterial lacZ gene was inserted into the gene such that the endogenous gene promoter drives expression of beta-galactosidase. While RT-PCR analysis detects a gene transcript in all tissues tested, strong beta-galactosidase expression from this allele is detectable only in a small region of the whole mount brainstem. No expression was detected on coronal sections of brain. Moderate staining was observed in the ovarian stroma.