A lacZ-neomycin cassette was inserted into the gene such that the endogenous gene promoter drives expression of beta-galactosidase and nucleotides from base 586 to 610 of exon 2 were deleted. RT-PCR analysis detects a high level of gene transcript in salivary gland and stomach, moderate levels in whole brain, cortex, subcortical region, cerebellum, brainstem, olfactory bulb, spinal cord, eye, Harderian glands, heart, kidney, lymph nodes, gallbladder, pituitary gland, adrenal gland, tongue, epididymis, coagulating gland, prostate gland and white fat and low levels in lung, liver, pancreas, spleen, thymus, bone marrow, skin, urinary bladder, skeletal muscle, small intestine, cecum, colon, testis, seminal vesicle, ovary and uterus. Beta-galactosidase expression from this allele is detectable in central nervous system, eyes, heart, kidney and cervix. Strong to very strong lacZ expression was detected throughout the whole mount brain as well as in sections of cerebrum, cerebellum, brainstem and spinal cord. In the eyes, very strong expression was detected in retina and ciliary body with moderate to strong expression in lens. Very strong staining was also observed throughout the myocardium. In kidney, very strong expression was detected throughout the cortex and medulla and in some cells of the papilla. The cervix shows very strong staining in the stratified squamous epithelium nearest the cervical canal. (J:156955)
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