将细菌的lacZ基因插入,使其由内源性基因驱动β-半乳糖苷酶的表达。RT-PCR检测结果显示在大脑、脊髓、脾、胸腺、淋巴结和膀胱中存在基因转录;在大脑和睾丸中能检测到β-半乳糖苷酶表达,但在脊髓、脾、胸腺、淋巴结和膀胱中未见。 (Translation from: A bacterial lacZ gene was inserted into the genome, with the endogenous promoter driving expression of beta-galactosidase. RT-PCR analysis revealed gene transcripts in brain, spinal cord, spleen, thymus, lymph nodes, and urinary bladder; beta-galactosidase expression was detected in brain and testis, but not in the spinal cord, spleen, thymus, lymph nodes, or urinary bladder, as per the source.)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129P2/OlaHsd
Targeted
插入
--
1
--
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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